PCR
From MyBio
This section of BioDirectory is primarily used by reseachers to design primers, and analyse those oligos to find out whether they'll form hairpin loops or primer dimers, and ascertain melt temperatures. There are also popular sections on PCR protocols this general section about PCR .
Polymerase chain reaction (PCR) has rapidly become one of the most widely used techniques in molecular biology and for good reason: it is a rapid, inexpensive and simple means of producing relatively large numbers of copies of DNA molecules from minute quantities of source DNA material--even when the source DNA is of relatively poor quality.
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PCR and multiplex PCR
Octavian Henegariu of Yale University wrote this guide and discussion of the main parameters determining the outcome of PCR reactions, including pictures, troubleshooting guide, standards and various applications.
